Inoculation Effect of Nitrogen-fixing and Phosphate-Solubilising Bacteria on Seed Germination of Brinjal (Solanum melongena L.)
Keywords:Plant Growth Promoting Traits, IAA, Phosphate Solubilisation, Germination %, SVI
Twenty rhizobacteria were isolated from rhizospheric soils of Uttarakhand Tarai region. Out of 20 isolates, 3
isolates (PB2, PB12, and PB19) were selected on the basis of morphological, biochemical, physiologically and
different PGP traits. The isolates were Gram negative, catalase positive, oxidase positive, starch and gelatine
hydrolysis positive. The bacterial cultures were positive for indole acetic acid production, phosphate
solubilisation, ammonia production, siderophore production, and nitrogen fixing activity. Isolate PB19 was the
best IAA producer strain (42.50μgml-1) while isolate PB2 was lowest IAA producer (25.34μgml-1)
comparatively. All the three isolates showed siderophore production and nitrogen fixing activity in G-NFMM
media. Effect of nitrogen fixing and phosphate solubilising PGPR alone and in different combinations was
studied on seed germination of brinjal plant. Treatments with different combination of bacterial isolates on
brinjal seeds showed maximum germination %, with consortia D (T8) and treatment 4 (PB19) by 95.83%
compared to control and other PGPR combinations after 8 days of incubation. The consortia of three isolated
bacteria showed significant effects on Seedling Vigour Index (SVI), germination index, and other associated
germination parameters. Thus, it was concluded that the use of combination of PGPR isolates could improve
seed germination and associated parameters of brinjal.
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