Formation of Dimers Composed of a Single Short dsDNA Connecting Two Gold Nanoparticles
DOI:
https://doi.org/10.13052/same2245-4551.114Keywords:
DNA, gold nanoparticles, gel electrophoresis.Abstract
We report synthesis of dimers composed of a single short double-stranded
(ds)DNA molecule connecting two gold nanoparticles (GNPs). Such struc-
tures may be useful for electrical transport measurements through dsDNA
molecules and for other research purposes. When the DNA molecules are
short with respect to the size of the GNP, gel electrophoresis cannot separ-
ate GNPs with different numbers of DNA molecules attached to them. We
present two methods to separate GNPs connected to single short thiolated
single-stranded (ss)DNA. The separation is performed by hybridizing the
DNA/GNP conjugates with long, partially complementary, ssDNA or with
complementary ssDNA connected to GNPs of smaller size. The separated
GNPs with a single short ssDNA were used to form dimers consisting of
GNPs connected by a single short dsDNA molecule.
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