Synthesis and Properties of Conjugates between Silver Nanoparticles and DNA-PNA Hybrids
DOI:
https://doi.org/10.13052/same2245-4551.113Keywords:
Silver nanoparticles, PNA-DNA hybrid, nanomaterials, TEM.Abstract
We describe the preparation and properties of a stable conjugate between two
15 nm silver nanoparticles (AgNPs) and a DNA-PNA hybrid composed of 10
guanine-cytosine base pairs. We show that the conjugate is spontaneously
formed during incubation of a DNA-PNA hybrid, containing phosphoro-
thioate residues at both ends of the DNA strand with AgNPs. The conjugate
molecules were separated from individual AgNPs and multiparticle structures
by gel electrophoresis. We demonstrate that the absorption spectrum of the
conjugate is broader than that of AgNPs, due to the interparticle plasmon
coupling.
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