Recruitment and retention of human autologous CD34+ CD117+ CD133+ bone marrow stem cells to infarcted myocardium followed by directed vasculogenesis: Novel strategy for cardiac regeneration

  • Marek Malecki Phoenix Biomolecular Engineering Foundation, San Francisco, CA, USA 2 NMRFM, National Institutes of Health, Madison, WI, USA 3 University of Wisconsin, Madison, WI, USA
  • Chelsea Sabo University of Wisconsin, Madison, WI, USA
  • Emily Putzer University of Wisconsin, Madison, WI, USA
  • Chris Stampe
  • Afsoon Foorohar
  • Carol Quach Phoenix Biomolecular Engineering Foundation, San Francisco, CA, USA
  • Michael Beauchaine
  • Xenia Tombokan
  • Mark Anderson NMRFM, National Institutes of Health, Madison, WI, USA
Keywords: Myocardial infarction, Regenerative medicine, Bone marrow stem cells, Stem cell therapy, Vasculogenesis, Heterospecific tetravalent antibodies

Abstract

Ongoing clinical trials, in regenerative therapy of patients suffering from myocardial infarctions, rely primarily upon administration of bone marrow stem cells to the infarcted zones. Unfortunately, low retention of these cells, to the therapeutic delivery sites, reduces effectiveness of this strategy; thus it has been identified as the most critical problem for advancement of cardiac regenerative medicine.

 

 

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Figure 1 Concept of novel strategy for cardiac regeneration with human autologous bone marrow cells. (A) Heterospecific, tetravalent antibodies (htAbs) contain binding domains for four different antigens: CD34, CD117, CD133, and myosin. They are injected into the solution flowing over the sarcomeres of the infarcted myocardium. (B) The htAbs dock onto myosins of the sarcomeres. Excess of the htAbs is cleared from the circulation. (C) Human, autologous, bone marrow stem cells (haBMSCs) are spiked into the solution. (D) The cells displaying on their surfaces CD34, CD117, CD133 are recruited and retained to the infarcted myocardium sarcomeres with the aid of the htAbs (only CD117 cell is shown here for clarity). The retained haBMSCs are directed to differentiate into endothelium with the defined factors (not shown).

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References

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Published
2013-12-13
Section
Research Article

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