Phytochemical Analysis, Antioxidant Activity and Acute Toxicity Study of Cicerarietinum and Brassica oleracea var. italica
Keywords:Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Diphenylpicrylhydrazyl (DPPH).
Plants have various bioactive components which are responsible for their different biological activities and
provides the basis for using plants for treatment of several diseases. The present study involves two different
plants Cicerarietinum and Brassica oleracea var. italica, locally available in Dehradun, Uttarakhand. Sprouts of
Cicerarietinum and leaves of Brassica oleracea var. italica were washed, air dried and then powdered. The
aqueous and methanolic extracts of these plants were used for the phytochemical anaysis. Besides
phytochemical assays, antioxidant activity of these plants were also done. Acute toxicity study was done on
Wistar rats to determine the toxicity level of these extracts.
The methanolic extract of Cicerarietinum sprouts (CAM) and aqueous extract of Cicerarietinum sprouts (CAA);
methanolic extract of Brassica oleracea var. italica leaves (BOM) aqueous extract of Brassica oleracea var.
italica leaves (BOM) possess various active constituents. CAA exhibit tannins, saponins and phenols, whereas
CAM possess tannins, saponins, alkaloids, flavonoids and phenols. In contrast, BOA and BOM both
containalkaloids, tannins, flavonoids and phenols. While saponins are present only in BOA. The TPC results
showed that BOM (247.79±0.710mg GAE g-1) has the highest phenolic content in it. In TFC analysis, BOA
showed the highest level of flavonoids (104.20±0.10mg QE g-1). In DPPH scavenging assay, BOM
(87.22±0.011) showed maximum % inhibition. After acute toxicity study plant extracts were found to be safe
even at highest concentration of 2000 mg kg-1
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