Influence of Age on Ginkgo Biloba Phytochemicals in Antimicrobial Activity Perspective
Ginkgo biloba is widely used for its pharmaceutical potential due to it’s medicinal value. Its medicinal properties are directly related to the presence of the phytochemical constituents, mainly the terpenes trilactone (ginkgolides and bilobalide) and flavonoid glycosides that are likely to be influenced by a number of soil, biogeographic and climatic factors as well as the age of the tree. The present study is aimed to investigate the influence of the age of Ginkgo tree with respect to the total phenolic and total flavonoid content, antioxidants and flavonoid glycosides along with the antimicrobial activity following Ultraviolet Spectroscopy and High Performance Liquid Chromatography.The results exhibited great variability in all the phytochemicals, under study, showing significant decline with ageing of the Ginkgo tree. However, the antimicrobial activity that was determined in terms of strong inhibition on a range of microorganisms (bacteria, actinomycetes and fungi) in plate assays, varied in this respect to limited extent.
Angioni, A., Barra, A., Coroneo, V., Dessi, S., & Cabras, P. (2006). Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. Stoechas essential oils from stem/ leaves and flowers, Journal of Agricultural and Food Chemistry, 54,4364-4370.
Barlow, S. M. (1990). Toxicological aspects of antioxidants used as food additives. In Food Antioxidants, Hudson BJF (ed.) Elsevier, London, pp 253-307.
Blum-Silva, C. H., Chaves, V. C., Schenkel, E. P., Coelho, G. C., & Reginatto, F. H. (2015). The influence of leaf age on methylxanthines, total phenolic content, and free radical scavenging capacity ofIlex paraguariensisaqueous extracts”, Revista Brasileira de Farmacognosia, 25(1), 1-6.
Bowers D., & Stamp, N. E. (1993). Effects of plant age, genotype and herbivory onPlantagoperformance and chemistry, Ecology, 74, 1778-1791.
Braquet, P., Esanu, A., Buisine, E., Hosford, D., Broquet, C., & Koltai, M. (1991). Recent progress in ginkgolide research, Medicinal Research Reviews, 11(3), 295-355.
Chang, C., Yang, M. H., Wen, H. M., & Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two comparative colorimetric methods, Journal of Food and Drug Analysis, 10(3), 178-182.
Doan, A., Ervin, G., Felton, G. (2004).Temporal effects on jasmonate induction of anti-herbivore defense inPhysalis angulata: seasonal and ontogenetic gradients, Biochemical Systematics and Ecology, 32(2), 117-126. Gopichand & Meena, R. L. (2015). Standardization of propagation and agro techniques inGinkgo bilobaL. -A medicinally important plant,Journal of Medicinal Plants Studies, 3(4), 6-15.
Harborne, J. B., & Williams, C. A. (2000). Advances in flavonoid research since 1992, Phytochemistry, 55(6), 481-504.
Hendriks, H., Anderson-Wildeboer, Y., Engels,G., Bos, R., & Woerdenbag, H. J. (1997). The content of parthenolide and its yield per plant during the growth ofTanacetumparthenium, Planta medica,63(4), 356-359.
Kaur,P., Chaudhary,A., Singh, B., & Gopichand. (2012). An efficient microwave assisted extraction of phenolic compounds and antioxidant potential of Ginkgo biloba,Natural Product Communication, 7(2), 203-206.
Kumar, A., Singh, S., & Pandey, A. (2009). General microflora, arbuscular mycorrhizal colonization and occurrence of endophytes in rhizosphere of two age groups ofGinkgobilobaL. of Indian central Himalaya, Indian Journal of Microbiology, 49, 134-141.
Mahadevan, S., & Park, Y. (2008). Multifaceted therapeutic benefits of Ginkgo biloba L.: chemistry, efficacy, safety and uses, Journalof Food Science, 73(1), 14-19.
Masotti, V., Juteau, F., Bessiere, J. M., & Viano, J. (2003). Seasonal and phenological variations of the essential oil from the narrow endemic species Artemisia molinieri and its biological activities,Journal of agricultural and food chemistry,51(24), 7115-7121.
Mazzanti, G., Mascellino, M. T., Battinelli, L., Coluccia, D., Manganaro, M., & Saso, L. (2000). Antimicrobial investigation of semipurified fractions of Ginkgo biloba leaves, Journal of Ethnopharmacology, 71, 83-88.
Nadeem, M., Rikhari, H. C., Kumar, A., Palni, L. M. S., Nandi, S. K. (2002).Taxol content in the bark of Himalayan Yew in relation to tree age and sex, Phytochemistry, 60, 627-631.
Pandey, A., & Agnihotri, V. (2015). Antimicrobials from Medicinal Plants: Research initiatives, Challenges and the Future prospects”, In: Biotechnology of Bioactive Compounds: Sources and Applications in Food and Pharmaceuticals (Eds. VK Gupta, M G Tuohy, A O‟Donovan, M Lohani), John Wiley & Sons, Ltd.
Pandey, A., Sati, P., Malviya, M. K., Singh, S., & Kumar A. (2014). Use of endophytic bacterium (Pseudomonas sp., MTCC9476) in propagation and conservation of Ginkgo biloba L.: A living fossil, Current Science.106(8), 1066-1067. Pandey, A., Singh, S., Kumar, A., Malviya, M. K., & Rinu, K. (2009). Isolation of an endophytic plant growth promoting bacterium Pseudomonassp. strain gb3 (MTCC 9476) fromGinkgobilobaL., growing in temperate Himalaya, NationalAcademy Science Letters, 32(3 & 4), 83-88.
Qian, D., Ju, J. M., Zhu, L.Y., Duan, J. A., Wang, Y. H., Zhang, S. J., & Guo, Q. S. (2002). Study on changing rules for total flavonoids and total terpene lactones in Ginkgo biloba leaves at different ages of tree and collecting seasons, Chinese Traditional and Herbal Drugs, 33(11), 1025-1027.
Rawat, S., Bhatt, I. D., & Rawal, R. S. (2011).Total phenolic compounds and antioxidant potential of Hedychium spicatum Butch. Ham. Ex D. Don in west Himalaya, India, Journal of Food Composition and Analysis, 24, 574-579.
Sati, P., Agnihotri, V., & Pandey, A. (2013). Optimization of temperature and time length during post chromatographic derivatization of TLC separation of ginkgolides and bilobalide standards, Journal of Planar Chromatography, 26(5), 452-454.
Sati, P., Pandey, A., & Palni, L. M. S. (2012). Antimicrobial potential of leaf extracts of Ginkgo biloba L., growing in Uttarakhand, Indian, National Academy Science Letters, 35(3), 201-206.
Sati, P., Pandey, A., Rawat, S., & Rani, A. (2013). Phytochemicals and antioxidants in leaf extracts of Ginkgo biloba with reference to location, seasonal variation and solvent system, Journal of Pharmacy Research, 7(9), 804-809.
Singh, B., Kaur, P., Gopichand, Singh, R. D., & Ahuja, P. S. (2008). Biologyand chemistry of Ginkgo biloba, Fitoterapia, 79, 401–418.
Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenol and other oxidation substrates and antioxidants by means of FolineCiocalteu reagent, Methods in Enzymology, 299, 152-178.
van Beek, T. A., & Montoro, P. (2009). Chemical analysis and quality control of Ginkgo biloba leaves, extracts, and phytopharmaceuticals. Journal of Chromatography A, 1216(11), 2002-2032.
Wang, X., Zhou, Z., Xu, F., & Xia, C. (1999). Concentration of Ginkgo flavnoids glycosides in Ginkgo biloba leaves in relation to the various season and tree’s age, Natural Product Research and Development, 12(2), 49-51.
Yao, X., Shang, E., Zhou, G., Tang, Y., Guo, S., Su, S., Jin, C., Qian, D., Qin, Y., & Duan, J. A. (2012). Comparative characterization oftotal flavonol glycosides and terpene lactones at different ages, from different cultivation sources and genders of Ginkgo biloba leaves, International Journal of Molecular Sciences, 13, 10305-10315.