Phytochemical Analysis, Antioxidant Activity and Acute Toxicity Study of Cicerarietinum and Brassica oleracea var. italica

  • Himani Badoni Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India
  • Promila Sharma Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, India
  • S. M. Waheed Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, India
  • Saumya Singh Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, India
Keywords: Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Diphenylpicrylhydrazyl(DPPH)


Plants have various bioactive components which are responsible for their different biological activities and provides the basis for using plants for treatment of several diseases. The present study involves two different plants Cicerarietinum and Brassica oleracea var. italica, locally available in Dehradun, Uttarakhand. Sprouts of Cicerarietinum and leaves of Brassica oleracea var. italica were washed, air dried and then powdered. The aqueous and methanolic extracts of these plants were used for the phytochemical anaysis. Besides phytochemical assays, antioxidant activity of these plants were also done. Acute toxicity study was done on Wistar rats to determine the toxicity level of these extracts.

The methanolic extract of Cicerarietinum sprouts(CAM) and aqueous extract of Cicerarietinum sprouts (CAA); methanolic extract of Brassica oleracea var. italica leaves (BOM) aqueous extract of Brassica oleracea var. italicaleaves (BOM) possess various active constituents.CAA exhibit tannins, saponins and phenols, whereas CAM possess tannins, saponins, alkaloids, flavonoids and phenols.In contrast, BOA and BOM both contain alkaloids, tannins, flavonoids and phenols. While saponins are present only in BOA. The TPC results showed that BOM (247.79±0.710mg GAE g-1) has the highest phenolic content in it. In TFC analysis, BOA showed the highest level of flavonoids (104.20±0.10mg QE g-1).In DPPH scavenging assay, BOM (87.22±0.011) showed maximum % inhibition. After acute toxicity study plant extracts were found to be safe even at highest concentration of 2000 mg kg-1.


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