Inoculation Effect of Nitrogen-fixing and Phosphate-Solubilising Bacteria on Seed Germination of Brinjal (Solanum melongena L.)

  • Tarun Sharma Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India
  • Navin Kumar Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India
  • Nishant Rai Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India
Keywords: Plant Growth Promoting Traits, IAA, Phosphate Solubilisation, Germination %, SVI


Twenty rhizobacteria were isolated from rhizospheric soils of Uttarakhand Tarai region. Out of 20 isolates, 3 isolates (PB2, PB12, and PB19) were selected on the basis of morphological, biochemical, physiologically and different PGP traits. The isolates were Gram negative, catalase positive, oxidase positive, starch and gelatine hydrolysis positive.The bacterial cultures were positive for indole acetic acid production, phosphate solubilisation, ammonia production, siderophore production, and nitrogen fixing activity. Isolate PB19 was the best IAA producer strain (42.50μgml-1) while isolate PB2 was lowest IAA producer (25.34μgml-1) comparatively. All the three isolates showed siderophore production and nitrogen fixing activity in G-NFMM media. Effect of nitrogen fixing and phosphate solubilising PGPR alone and in different combinations was studied on seed germination of brinjal plant. Treatments with different combination of bacterial isolates on brinjal seeds showed maximum germination %, with consortia D (T8) and treatment 4 (PB19) by 95.83% compared to control and other PGPR combinations after 8 days of incubation.The consortia of three isolated bacteria showed significant effects on Seedling Vigour Index (SVI), germination index, and other associated germination parameters. Thus, it was concluded that the use of combination of PGPR isolates could improve seed germination and associated parameters of brinjal.


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