Dexamethasone Induced Modulation of Reproduction: Investigating Role of Dexras1 Monomeric G Protein

  • Rashmi Verma Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India
  • Navin Kumar Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India
  • Ashish Thapliyal Department of Life Sciences, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India
Keywords: Reproduction, Dexamethasone, Dexras1, Stress, LH, FSH, Glucocorticoids

Abstract

Many factors like hormonal, anatomical, genetic, and environmental etc effect reproduction. Stressful conditions like emotional-stress, altered level of hormones etc can cause alterations in release of reproductive hormones thus ultimately effecting reproduction. In our study, we have focused to know the modulation of reproduction induced by Dexamethasone(DEX)(a synthetic glucocorticoid), which mimics as a stress conditionin female miceand investigated the role of Dexras1 in reproductionunder the influence of Dexamethasone. Glucocorticoid are known toinfluence the release of GnRH hormone which in turn regulate the release of reproductive hormones(LH, FSH). Dexamethasone is reported to upregulates Dexras1 expression. Sowe have investigatedthe modulation of reproduction by alteration in the release of reproductive hormones LH and FSH under DEX dosage in female mice. We observed that the level of hormones LH and FSH altered, in the presence of Dexamethasone doses (0.1 and 0.5mg/kg mice). LH level is decreased while the level of FSH increasedwith DEX dose. So the present study reveals the role of Dexamethasone in modulation of reproduction. It has beenreported earlier that Dexras1 is upregulated by Dexamethasone so we couldsuggest that Dexras1 might involvein modulation of reproduction and could become a very important therapeutic target in reproduction signaling processes.

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References

Breen, K. M., & Mellon, P. L. (2014). Influence of stress-induced intermediates on gonadotropin gene expression in gonadotrope cells. Molecular and Cellular Endocrinology, 385, 71–77.

Breen, K. M., Thackray, V. G., Hsu, T., Mak-McCully, R. A., Coss, D., & Mellon, P. L. (2012). Stress levels of glucocorticoids inhibit LHβ-subunit gene expression in gonadotrope cells.Molecular Endocrinology, 26(10), 1716-1731.

Dardes, R. C., Baracat, E. C., & Simões, M. J. (2000). Modulation of estrous cycle and LH, FSH and melatonin levels by pinealectomy and sham-pinealectomy in female rats.Progress in Neuro-Psychopharmacology & Biological Psychiatry, 24(3), 441-453.

Dolatabadi, A. A., & Zarchii, S. R. (2015). The effect of prescription of different dexamethasone doses on reproductive system. Biomedical Research, 26(4), 656-660.

Fernandez, I., Pena, A., Del Teso, N., Perez, V., & Rodriguez-Cuesta, J. (2010). Clinical biochemistry parameters in C57BL/6J mice afterblood collection from the submandibular vein and retroorbital plexus. Journal of the American Association for Laboratory Animal Science, 49(2), 202–206.

Gao, H., Gao, Y., Li, X., Shen, A., & Yan, M. (2010). Spatiotemporal patterns of dexamethasone-induced Ras protein 1 expression in the central nervous system of rats with experimental autoimmune encephalomyelitis. Journal of Molecular Neuroscience, 41(1), 198-209.

Gooyande, J., Modaresi, M., & Pirestani, A. (2014). Comparing betamethasone and dexamethasone effects on concentration of male reproductive hormones in mice. Research Journal of Applied Sciences, Engineering and Technology, 7(2), 413-416.

Gore, A. C., Attardi, B.,&DeFranco, D. B. (2006). Glucocorticoid repression of the reproductive axis: effects on GnRH and gonadotropin subunit mRNA levels.Molecular and Cellular Endocrinology, 256(1-2), 40-48.Hong, K., & Choi, Y. (2018). Role of estrogen and RAS signaling in repeated implantation failure. BMB Reports,51(5), 225-229. Kemppainen, R. J., & Behrend, E. N. (1998). Dexamethasone rapidly induces a novel ras superfamily member-related gene in AtT-20 cells. Journal of Biological Chemistry, 273(6), 3129–3131.

Kim, H. R., Cho, K. S., Kim, E., Lee, O. H., Yoon, H., Lee, S., Moon, S., Park, M., Hong, K., Na, Y., Shin, J.E., Kwon, H., Song, H., Choi, D.H., & Choi, Y. (2017). Rapid expression of RASD1 is regulated by estrogen receptor-dependent intracellular signaling pathway in the mouse uterus. Molecular and Cellular Endocrinology, 446, 32-39.

Li, X., Cheng, C., Fei, M., Gao, S., Niu, S., Chen, M., Liu, Y., Guo, Z., Wang, H., Zhao, J., Yu, X., & Shen, A. (2008). Spatiotemporal expression of Dexras1 after spinal cord transection in rats. Cellular & Molecular Neurobiology, 28(3), 371–388.

Schafer, S. C., Wallerath, T., Closs, E. I., Schmidt, C., Schwarz, P. M., Forstermann, U., & Lehr, H. A. (2005). Dexamethasone suppresses eNOS and CAT-1 and induces moxidative stress in mouse resistance arterioles. American Journal of Physiology-Heart and Circulatory Physiology, 288, H436–H444.

Shirasaki, Y., Ito, Y., Kikuchi, M., Imamura, Y., & Hayashi, T. (2012). Validation studies on blood collection from the jugular vein of conscious mice.Journal of the American Association for Laboratory Animal Science, 51(3), 345–351.

Soga, T., Dalpatadu, S. L., Wong, D. W., & Parhar, I. S. (2012). Neonatal dexamethasone exposure down-regulates GnRH expression through the GnIH pathway in female mice. Neuroscience, 218, 56-64.

Published
2020-03-09
Section
Articles